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High-performance quantification of mature microRNAs by real-time RT-PCR using deoxyuridine-incorporated oligonucleotides and hemi-nested primers

MicroRNAs are small noncoding RNAs that serve as important regulators of eukaryotic gene expression and are emerging as novel diagnostic and therapeutic targets for human diseases. Robust and reliable detection of miRNAs is an essential step for understanding the functional significance of these sma...

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Bibliografski detalji
Glavni autori: Wan, Guoqiang, ‘En Lim, Qing, Too, Heng-Phon
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885692/
https://ncbi.nlm.nih.gov/pubmed/20547774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2001610
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