In Vivo Identification of Sumoylation Sites by a Signature Tag and Cysteine-targeted Affinity Purification

Small ubiquitin-like modifier (SUMO) is conjugated to its substrates via an enzymatic cascade consisting of three enzymes, E1, E2, and E3. The active site of the E2 enzyme, Ubc9, recognizes the substrate through binding to a consensus tetrapeptide ΨKXE. However, recent proteomics studies suggested t...

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Autores principales: Blomster, Henri A., Imanishi(今西, Susumu Y., 進), Siimes, Jenny, Kastu, Juha, Morrice, Nick A., Eriksson, John E., Sistonen, Lea
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2010
Materias:
Genomics and Proteomics
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885211/
https://ncbi.nlm.nih.gov/pubmed/20388717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.106955
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