In Vivo Identification of Sumoylation Sites by a Signature Tag and Cysteine-targeted Affinity Purification

Small ubiquitin-like modifier (SUMO) is conjugated to its substrates via an enzymatic cascade consisting of three enzymes, E1, E2, and E3. The active site of the E2 enzyme, Ubc9, recognizes the substrate through binding to a consensus tetrapeptide ΨKXE. However, recent proteomics studies suggested t...

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Autors principals: Blomster, Henri A., Imanishi(今西, Susumu Y., 進), Siimes, Jenny, Kastu, Juha, Morrice, Nick A., Eriksson, John E., Sistonen, Lea
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2010
Matèries:
Genomics and Proteomics
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885211/
https://ncbi.nlm.nih.gov/pubmed/20388717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.106955
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