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In Vivo Identification of Sumoylation Sites by a Signature Tag and Cysteine-targeted Affinity Purification
Small ubiquitin-like modifier (SUMO) is conjugated to its substrates via an enzymatic cascade consisting of three enzymes, E1, E2, and E3. The active site of the E2 enzyme, Ubc9, recognizes the substrate through binding to a consensus tetrapeptide ΨKXE. However, recent proteomics studies suggested t...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2885211/ https://ncbi.nlm.nih.gov/pubmed/20388717 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.106955 |
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