Improving Molecular Detection of Fungal DNA in Formalin-Fixed Paraffin-Embedded Tissues: Comparison of Five Tissue DNA Extraction Methods Using Panfungal PCR

DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues is difficult and requires special protocols in order to extract small amounts of DNA suitable for amplification. Most described methods report an amplification success rate between 60 and 80%; therefore, there is a need to improve m...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Muñoz-Cadavid, C., Rudd, S., Zaki, S. R., Patel, M., Moser, S. A., Brandt, M. E., Gómez, B. L.
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology (ASM) 2010
נושאים:
Mycology
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2884522/
https://ncbi.nlm.nih.gov/pubmed/20392915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00459-10
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