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Optimizing Transfection of Primary Human Umbilical Vein Endothelial Cells Using Commercially Available Chemical Transfection Reagents

Primary cells, such as HUVEC, are notoriously difficult to transfect and are susceptible to the toxic effects of transfection reagents. A transfection reagent with a high transfection efficiency and low cytotoxicity was sought to retain sufficient viability of transfected HUVEC for subsequent assays...

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Detalhes bibliográficos
Main Authors: Hunt, Michelle A., Currie, Margaret J., Robinson, Bridget A., Dachs, Gabi U.
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2884313/
https://ncbi.nlm.nih.gov/pubmed/20592869
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