Optimizing Transfection of Primary Human Umbilical Vein Endothelial Cells Using Commercially Available Chemical Transfection Reagents

Primary cells, such as HUVEC, are notoriously difficult to transfect and are susceptible to the toxic effects of transfection reagents. A transfection reagent with a high transfection efficiency and low cytotoxicity was sought to retain sufficient viability of transfected HUVEC for subsequent assays...

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Bibliographische Detailangaben
Hauptverfasser: Hunt, Michelle A., Currie, Margaret J., Robinson, Bridget A., Dachs, Gabi U.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Association of Biomolecular Resource Facilities 2010
Schlagworte:
Communications
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2884313/
https://ncbi.nlm.nih.gov/pubmed/20592869
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