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Optimizing Transfection of Primary Human Umbilical Vein Endothelial Cells Using Commercially Available Chemical Transfection Reagents

Primary cells, such as HUVEC, are notoriously difficult to transfect and are susceptible to the toxic effects of transfection reagents. A transfection reagent with a high transfection efficiency and low cytotoxicity was sought to retain sufficient viability of transfected HUVEC for subsequent assays...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Hunt, Michelle A., Currie, Margaret J., Robinson, Bridget A., Dachs, Gabi U.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Association of Biomolecular Resource Facilities 2010
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2884313/
https://ncbi.nlm.nih.gov/pubmed/20592869
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