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Electrochemical DNA Detection via Exonuclease and Target-Catalyzed Transformation of Surface-Bound Probes

We report a single-step, single reagent, label-free, isothermal electrochemical DNA sensor based on the phenomenon of target-recycling. The sensor exploits strand-specific exonuclease activity to achieve selective enzymatic digestion of target/probe duplexes. This results in a permanent change in th...

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Detalhes bibliográficos
Main Authors: Hsieh, Kuangwen, Xiao, Yi, Soh, H. Tom
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2883016/
https://ncbi.nlm.nih.gov/pubmed/20232926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/la100227s
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