טוען...
Targeted gene deletions in C. elegans using transposon excision
We have developed a method, MosDel, to generate targeted knock-outs of genes in C. elegans. We make use of the Mos1 transposase to excise a Mos1 transposon adjacent to the region to be deleted. The double-strand break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA...
שמור ב:
Main Authors: | , , , , , , , , , , |
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פורמט: | Artigo |
שפה: | Inglês |
יצא לאור: |
2010
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נושאים: | |
גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2878396/ https://ncbi.nlm.nih.gov/pubmed/20418868 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1454 |
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