Targeted gene deletions in C. elegans using transposon excision

We have developed a method, MosDel, to generate targeted knock-outs of genes in C. elegans. We make use of the Mos1 transposase to excise a Mos1 transposon adjacent to the region to be deleted. The double-strand break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA...

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Auteurs principaux: Frøkjær-Jensen, Christian, Davis, M. Wayne, Hollopeter, Gunther, Taylor, Jon, Harris, Todd, Nix, Paola, Lofgren, Rachel, Prestgard-Duke, Michael, Bastiani, Michael, Moerman, Donald G., Jorgensen, Erik M.
Format: Artigo
Langue:Inglês
Publié: 2010
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2878396/
https://ncbi.nlm.nih.gov/pubmed/20418868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1454
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