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Mammalian nuclear transplantation to Germinal Vesicle stage Xenopus oocytes – A method for quantitative transcriptional reprogramming

Full-grown Xenopus oocytes in first meiotic prophase contain an immensely enlarged nucleus, the Germinal Vesicle (GV), that can be injected with several hundred somatic cell nuclei. When the nuclei of mammalian somatic cells or cultured cell lines are injected into a GV, a wide range of genes that a...

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Bibliografiska uppgifter
Huvudupphovsmän: Halley-Stott, R.P., Pasque, V., Astrand, C., Miyamoto, K., Simeoni, I., Jullien, J., Gurdon, J.B.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Academic Press 2010
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2877800/
https://ncbi.nlm.nih.gov/pubmed/20123126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.01.035
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