A Cellular Screening Assay Using Analysis of Metal-Modified Fluorescence Lifetime

Current methods for screening cell receptor internalization often require complex image analysis with limited sensitivity. Here we describe a novel bioassay based on detection of changes in global fluorescence lifetime above a gold substrate, with superresolution axial sensitivity and no need for im...

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Bibliografiske detaljer
Main Authors: Cade, Nicholas I., Fruhwirth, Gilbert, Archibald, Stephen J., Ng, Tony, Richards, David
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2010
Fag:
Spectroscopy, Imaging, and Other Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2877351/
https://ncbi.nlm.nih.gov/pubmed/20513420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.03.016
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