Quantitative Lifetime Unmixing of Multiexponentially Decaying Fluorophores Using Single-Frequency Fluorescence Lifetime Imaging Microscopy

Fluorescence lifetime imaging microscopy (FLIM) is a quantitative microscopy technique for imaging nanosecond decay times of fluorophores. In the case of frequency-domain FLIM, several methods have been described to resolve the relative abundance of two fluorescent species with different fluorescenc...

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Библиографические подробности
Главные авторы: Kremers, Gert-Jan, van Munster, Erik B., Goedhart, Joachim, Gadella, Theodorus W. J.
Формат: Artigo
Язык:Inglês
Опубликовано: The Biophysical Society 2008
Предметы:
Spectroscopy, Imaging, Other Techniques
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2426628/
https://ncbi.nlm.nih.gov/pubmed/18359789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.125229
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