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Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis

This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologicall...

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Detalhes bibliográficos
Main Authors: Ye, Shijie, Ann Berger, Allison, Petzold, Dominique, Reimann, Oliver, Matt, Benjamin, Koksch, Beate
Formato: Artigo
Idioma:Inglês
Publicado em: Beilstein-Institut 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2874332/
https://ncbi.nlm.nih.gov/pubmed/20502658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3762/bjoc.6.40
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