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Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis
This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologicall...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Beilstein-Institut
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2874332/ https://ncbi.nlm.nih.gov/pubmed/20502658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3762/bjoc.6.40 |
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