Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin.
A theory for single-molecule fluorescence detection is developed and then used to analyze data from subpicomolar solutions of B-phycoerythrin (PE). The distribution of detected counts is the convolution of a Poissonian continuous background with bursts arising from the passage of individual fluoroph...
Збережено в:
| Опубліковано в:: | Proc Natl Acad Sci U S A |
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| Автори: | , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
National Academy of Sciences
1989
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287394/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2726766/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.11.4087 |
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