Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin.

A theory for single-molecule fluorescence detection is developed and then used to analyze data from subpicomolar solutions of B-phycoerythrin (PE). The distribution of detected counts is the convolution of a Poissonian continuous background with bursts arising from the passage of individual fluoroph...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Peck, K, Stryer, L, Glazer, A N, Mathies, R A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1989
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287394/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2726766/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.11.4087
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