Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay

The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used Salmonella enrichment broths, Rappaport-Vassiliadis (RV) and Muller-Kauffmann tetrathionate with...

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Detalhes bibliográficos
Main Authors: Hyeon, Ji-Yeon, Hwang, In-Gyun, Kwak, Hyo-Sun, Park, Chankyu, Choi, In-Soo, Seo, Kun-Ho
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Society of Veterinary Science 2010
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2873814/
https://ncbi.nlm.nih.gov/pubmed/20458155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4142/jvs.2010.11.2.143
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