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A cell line producing recombinant nerve growth factor evokes growth responses in intrinsic and grafted central cholinergic neurons.
The rat beta nerve growth factor (NGF) gene was inserted into a mammalian expression vector and cotransfected with a plasmid conferring resistance to neomycin into mouse 3T3 fibroblasts. From this transfection a stable cell line was selected that contains several hundred copies of the rat NGF gene a...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287352/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2734317/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.12.4756 |
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