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Comparison between Whole Distribution- and Average-Based Approaches to the Determination of Fluorescence Resonance Energy Transfer Efficiency in Ensembles of Proteins in Living Cells

Current methods for analysis of data from studies of protein-protein interactions using fluorescence resonance energy transfer (FRET) emerged from several decades of research using wide-field microscopes and spectrofluorometers to measure fluorescence from individual cells or cell populations. Inher...

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Main Authors: Singh, Deo R., Raicu, Valerică
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2872365/
https://ncbi.nlm.nih.gov/pubmed/20483320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.01.048
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