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Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer
We describe a fluorescence resonance energy transfer (FRET)-based method for finding in living cells the fraction of a protein population (α(T)) forming complexes, and the average number (n) of those protein molecules in each complex. The method relies both on sensitized acceptor emission and on don...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Portland Press Ltd.
2004
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1134695/ https://ncbi.nlm.nih.gov/pubmed/15352875 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20040226 |
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