Peptide sequencing and site-directed mutagenesis identify tyrosine-727 as the active site tyrosine of Saccharomyces cerevisiae DNA topoisomerase I.

Extensive digestion of the covalent intermediate between DNA and Saccharomyces cerevisiae DNA topoisomerase I with trypsin yields a 7-amino acid peptide covalently linked to DNA. Direct sequencing of the DNA-linked peptide identifies Tyr-727 as the active site tyrosine that forms an O4-phosphotyrosi...

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發表在:	Proc Natl Acad Sci U S A
Principais autores:	Lynn, R M, Bjornsti, M A, Caron, P R, Wang, J C
格式:	Artigo
語言:	Inglês
出版:	National Academy of Sciences 1989
主題:	Research Article
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287177/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2542938/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.10.3559
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