Peptide sequencing and site-directed mutagenesis identify tyrosine-727 as the active site tyrosine of Saccharomyces cerevisiae DNA topoisomerase I.

Extensive digestion of the covalent intermediate between DNA and Saccharomyces cerevisiae DNA topoisomerase I with trypsin yields a 7-amino acid peptide covalently linked to DNA. Direct sequencing of the DNA-linked peptide identifies Tyr-727 as the active site tyrosine that forms an O4-phosphotyrosi...

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Main Authors: Lynn, R M, Bjornsti, M A, Caron, P R, Wang, J C
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC287177/
https://ncbi.nlm.nih.gov/pubmed/2542938
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