A Two-Tube Combined TaqMan/SYBR Green Assay to Identify Mycobacteria and Detect Single Global Lineage-Defining Polymorphisms in Mycobacterium tuberculosis

We have developed a novel real-time PCR assay to identify and perform preliminary genotyping of mycobacteria in a manner tailored to our local service. Within a single thermocycler run, mycobacterial 16S rDNA and the Mycobacterium tuberculosis global lineage-defining RD750 polymorphism are targeted...

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Detalhes bibliográficos
Main Authors: Cheah, Eddy S.G., Malkin, Joanne, Free, Robert C., Lee, Su-Min, Perera, Nelun, Woltmann, Gerrit, Patel, Hemu, Kimmitt, Patrick T., Smith, Rebecca J., Rajakumar, Kumar, Barer, Michael R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2010
Assuntos:
Regular Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2871733/
https://ncbi.nlm.nih.gov/pubmed/20093392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090030
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