A Lentiviral Functional Proteomics Approach Identifies Chromatin Remodeling Complexes Important for the Induction of Pluripotency

Protein complexes and protein-protein interactions are essential for almost all cellular processes. Here, we establish a mammalian affinity purification and lentiviral expression (MAPLE) system for characterizing the subunit compositions of protein complexes. The system is flexible (i.e. multiple N-...

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Detalhes bibliográficos
Main Authors: Mak, Anthony B., Ni, Zuyao, Hewel, Johannes A., Chen, Ginny I., Zhong, Guoqing, Karamboulas, Konstantina, Blakely, Kim, Smiley, Sandra, Marcon, Edyta, Roudeva, Denitza, Li, Joyce, Olsen, Jonathan B., Wan, Cuihong, Punna, Thanuja, Isserlin, Ruth, Chetyrkin, Sergei, Gingras, Anne-Claude, Emili, Andrew, Greenblatt, Jack, Moffat, Jason
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Stem Cell Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2871416/
https://ncbi.nlm.nih.gov/pubmed/20305087
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M000002-MCP201
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