Transfer of the inducible lac repressor/operator system from Escherichia coli to a vaccinia virus expression vector.
Cis- and trans-acting elements of the Escherichia coli lac operon were transferred to vaccinia virus and used to regulate gene expression. A recombinant virus that constitutively expresses a modified lac repressor gene (lacI) was constructed. We calculated that each infected cell contained approxima...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1989
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286954/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2649884/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.8.2549 |
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