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Transfer of the inducible lac repressor/operator system from Escherichia coli to a vaccinia virus expression vector.

Cis- and trans-acting elements of the Escherichia coli lac operon were transferred to vaccinia virus and used to regulate gene expression. A recombinant virus that constitutively expresses a modified lac repressor gene (lacI) was constructed. We calculated that each infected cell contained approxima...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Fuerst, T R, Fernandez, M P, Moss, B
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286954/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2649884/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.8.2549
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