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Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography.

Immobilized metal ion affinity chromatography (IMAC) has been explored as a probe into the topography of histidyl residues of a protein molecule. An evaluation of the chromatographic behavior of selected model proteins--thioredoxin, ubiquitin, calmodulin, lysozyme, cytochrome c, and myoglobin on imm...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Hemdan, E S, Zhao, Y J, Sulkowski, E, Porath, J
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1989
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286794/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2538816/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.6.1811
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