Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell

We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below −170 °C, artifacts due to dehydration, ice crystallization, and radiation damag...

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Main Authors: Huang, Xiaojing, Nelson, Johanna, Kirz, Janos, Lima, Enju, Marchesini, Stefano, Miao, Huijie, Neiman, Aaron M., Shapiro, David, Steinbrener, Jan, Stewart, Andrew, Turner, Joshua J., Jacobsen, Chris
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2866741/
https://ncbi.nlm.nih.gov/pubmed/20365955
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