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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
This protocol describes the growth and stimulation, with the fatty acid oleate, of isotopically heavy and light S. cerevisiae cells. Cells are ground using a cryolysis procedure in a ball mill grinder and the resulting grindate brought into solution by urea solubilization. This procedure allows for...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2865974/ https://ncbi.nlm.nih.gov/pubmed/19823167 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1474 |
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