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Aggregation of a multidomain protein: A coagulation mechanism governs aggregation of a model IgG1 antibody under weak thermal stress

Using an IgG1 antibody as a model system, we have studied the mechanisms by which multidomain proteins aggregate at physiological pH when incubated at temperatures just below their lowest thermal transition. In this temperature interval, only minor changes to the protein conformation are observed. L...

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Detalhes bibliográficos
Main Authors: Andersen, Christian Beyschau, Manno, Mauro, Rischel, Christian, Thórólfsson, Matthías, Martorana, Vincenzo
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2865720/
https://ncbi.nlm.nih.gov/pubmed/20014440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.309
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