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Aggregation of a multidomain protein: A coagulation mechanism governs aggregation of a model IgG1 antibody under weak thermal stress

Using an IgG1 antibody as a model system, we have studied the mechanisms by which multidomain proteins aggregate at physiological pH when incubated at temperatures just below their lowest thermal transition. In this temperature interval, only minor changes to the protein conformation are observed. L...

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Autors principals: Andersen, Christian Beyschau, Manno, Mauro, Rischel, Christian, Thórólfsson, Matthías, Martorana, Vincenzo
Format: Artigo
Idioma:Inglês
Publicat: Wiley Subscription Services, Inc., A Wiley Company 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2865720/
https://ncbi.nlm.nih.gov/pubmed/20014440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.309
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