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Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX

AlgX is a periplasmic protein required for the production of the exopoly­saccharide alginate in Pseudomonas sp. and Azotobacter vinelandii. AlgX has been overexpressed and purified and diffraction-quality crystals have been grown using iterative seeding and the hanging-drop vapor-diffusion method. T...

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Main Authors: Weadge, Joel T., Yip, Patrick P., Robinson, Howard, Arnett, Krista, Tipton, Peter A., Howell, P. Lynne
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864699/
https://ncbi.nlm.nih.gov/pubmed/20445266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110011851
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