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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyoxalase I from Leishmania infantum

Glyoxalase I (GLO1) is the first of the two glyoxalase-pathway enzymes. It catalyzes the formation of S-d-lactoyltrypanothione from the non-enzymatically formed hemithioacetal of methylglyoxal and reduced trypanothione. In order to understand its substrate binding and catalytic mechanism, GLO1 from...

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主要な著者: Barata, Lídia, Sousa Silva, Marta, Schuldt, Linda, da Costa, Gonçalo, Tomás, Ana M., Ferreira, António E. N., Weiss, Manfred S., Ponces Freire, Ana, Cordeiro, Carlos
フォーマット: Artigo
言語:Inglês
出版事項: International Union of Crystallography 2010
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864695/
https://ncbi.nlm.nih.gov/pubmed/20445262
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110010754
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