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A preliminary crystallographic study of recombinant NicX, an Fe(2+)-dependent 2,5-dihydroxypyridine dioxygenase from Pseudomonas putida KT2440

NicX from Pseudomonas putida KT2440 is an Fe(2+)-dependent dioxygenase that is involved in the aerobic degradation of nicotinic acid. The enzyme converts 2,5-­dihydroxypyridine to N-formylmaleamic acid when overexpressed in Escherichia coli. Biophysical characterization of NicX by analytical gel-fil...

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Detalhes bibliográficos
Main Authors: Jiménez, José Ignacio, Acebrón, Iván, García, José Luis, Díaz, Eduardo, Mancheño, José Miguel
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864690/
https://ncbi.nlm.nih.gov/pubmed/20445257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911001119X
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