Single-Step Affinity Purification for Fungal Proteomics

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches,...

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Detalhes bibliográficos
Main Authors: Liu, Hui-Lin, Osmani, Aysha H., Ukil, Leena, Son, Sunghun, Markossian, Sarine, Shen, Kuo-Fang, Govindaraghavan, Meera, Varadaraj, Archana, Hashmi, Shahr B., De Souza, Colin P., Osmani, Stephen A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2010
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2863959/
https://ncbi.nlm.nih.gov/pubmed/20363899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00032-10
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