Single-Step Affinity Purification for Fungal Proteomics

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches,...

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Autori principali: Liu, Hui-Lin, Osmani, Aysha H., Ukil, Leena, Son, Sunghun, Markossian, Sarine, Shen, Kuo-Fang, Govindaraghavan, Meera, Varadaraj, Archana, Hashmi, Shahr B., De Souza, Colin P., Osmani, Stephen A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2010
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Articles
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2863959/
https://ncbi.nlm.nih.gov/pubmed/20363899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00032-10
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