Protein-DNA chimeras for single molecule mechanical folding studies with the optical tweezers

Here we report on a method that extends the study of the mechanical behavior of single proteins to the low force regime of optical tweezers. This experimental approach relies on the use of DNA handles to specifically attach the protein to polystyrene beads and minimize the non-specific interactions...

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Bibliographic Details
Main Authors: Cecconi, Ciro, Shank, Elizabeth A., Dahlquist, Frederick W., Marqusee, Susan, Bustamante, Carlos
Format: Artigo
Language:Inglês
Published: 2008
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2862981/
https://ncbi.nlm.nih.gov/pubmed/18183383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00249-007-0247-y
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