SeqSharp: A General Approach for Improving Cycle-Sequencing That Facilitates a Robust One-Step Combined Amplification and Sequencing Method

Sequencing a specific DNA element within a genome or a complex mixture of DNA by the Sanger sequencing method generally involves PCR-mediated amplification of target DNA with forward and reverse primers, followed by a sequencing reaction directed from a single primer. To minimize the contribution of...

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Hlavní autoři: SenGupta, Dhruba J., Cookson, Brad T.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Investigative Pathology 2010
Témata:
Technical Advances
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860461/
https://ncbi.nlm.nih.gov/pubmed/20203000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090134
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