A Tagging-via-substrate Approach to Detect the Farnesylated Proteome Using Two-dimensional Electrophoresis Coupled with Western Blotting

Prenylation is a post-translational modification critical for the proper function of multiple physiologically important proteins, including small G-proteins, such as Ras. Methods allowing rapid and selective detection of protein farnesylation and geranylgeranylation are fundamental for the understan...

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Detalhes bibliográficos
Main Authors: Onono, Fredrick O., Morgan, Michael A., Spielmann, H. Peter, Andres, Douglas A., Subramanian, Thangaiah, Ganser, Arnold, Reuter, Christoph W. M.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860237/
https://ncbi.nlm.nih.gov/pubmed/20103566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900597-MCP200
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