Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

We have achieved the ability to synthesize thousands of unique, long oligonucleotides (150mers) in fmol amounts using parallel synthesis of DNA on microarrays. The sequence accuracy of the oligonucleotides in such large-scale syntheses has been limited by the yields and side reactions of the DNA syn...

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Главные авторы: LeProust, Emily M., Peck, Bill J., Spirin, Konstantin, McCuen, Heather Brummel, Moore, Bridget, Namsaraev, Eugeni, Caruthers, Marvin H.
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2010
Предметы:
Synthetic Biology and Chemistry
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860131/
https://ncbi.nlm.nih.gov/pubmed/20308161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq163
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