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Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process
We have achieved the ability to synthesize thousands of unique, long oligonucleotides (150mers) in fmol amounts using parallel synthesis of DNA on microarrays. The sequence accuracy of the oligonucleotides in such large-scale syntheses has been limited by the yields and side reactions of the DNA syn...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2860131/ https://ncbi.nlm.nih.gov/pubmed/20308161 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq163 |
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