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Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method

The stable cloning of giant DNA is a necessary process in the production of recombinant/synthetic genomes. Handling DNA molecules in test tubes becomes increasingly difficult as their size increases, particularly above 100 kb. The need to prepare such large DNA molecules in a regular manner has limi...

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Detalles Bibliográficos
Main Authors: Itaya, Mitsuhiro, Kaneko, Shinya
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860128/
https://ncbi.nlm.nih.gov/pubmed/20308163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq142
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