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An inducible recA expression Bacillus subtilis genome vector for stable manipulation of large DNA fragments

BACKGROUND: The Bacillus subtilis genome (BGM) vector is a novel cloning system based on the natural competence that enables B. subtilis to import extracellular DNA fragments into the cell and incorporate the recombinogenic DNA into the genome vector by homologous recombination. The BGM vector syste...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Ogawa, Takafumi, Iwata, Tetsuo, Kaneko, Shinya, Itaya, Mitsuhiro, Hirota, Junji
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4374399/
https://ncbi.nlm.nih.gov/pubmed/25879542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-015-1425-4
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