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Galectin-1 as a fusion partner for the production of soluble and folded human β-1,4-galactosyltransferase-T7 in E. coli

The expression of recombinant proteins in E. coli often leads to inactive aggregated proteins known as the inclusion bodies. To date, the best available tool has been the use of fusion tags, including the carbohydrate-binding protein; e.g., the maltose-binding protein (MBP) that enhances the solubil...

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Hlavní autoři: Pasek, Marta, Boeggeman, Elizabeth, Ramakrishnan, Boopathy, Qasba, Pradman K.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2859968/
https://ncbi.nlm.nih.gov/pubmed/20226765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.03.051
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