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Site-specific labeling of nucleotides for making RNA for high resolution NMR studies using an E. coli strain disabled in the oxidative pentose phosphate pathway

Escherichia coli (E. coli) is a versatile organism for making nucleotides labeled with stable isotopes ((13)C, (15)N, and/or (2)H) for structural and molecular dynamics characterizations. Growth of a mutant E. coli strain deficient in the pentose phosphate pathway enzyme glucose-6-phosphate dehydrog...

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書目詳細資料
Main Authors: Dayie, T. Kwaku, Thakur, Chandar S.
格式: Artigo
語言:Inglês
出版: Springer Netherlands 2010
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2859161/
https://ncbi.nlm.nih.gov/pubmed/20309608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-010-9405-0
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