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Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

A composite plasmid has been constructed in vitro from colicin E1 factor (mass of 4.2 megadaltons [Md]) and nontransmissible resistance factor RSF 1010 (mass, 5.5. Md) deoxyribonucleic acids (DNAs) by the sequential action of Escherichia coli endonuclease (RI (Eco RI) and T4 phage DNA ligase on the...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Main Authors: Tanaka, T, Weisblum, B
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC285650/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1090574/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.121.1.354-362.1975
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