Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
A composite plasmid has been constructed in vitro from colicin E1 factor (mass of 4.2 megadaltons [Md]) and nontransmissible resistance factor RSF 1010 (mass, 5.5. Md) deoxyribonucleic acids (DNAs) by the sequential action of Escherichia coli endonuclease (RI (Eco RI) and T4 phage DNA ligase on the...
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| Published in: | J Bacteriol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1975
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC285650/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1090574/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.121.1.354-362.1975 |
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