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Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes

RNA interference (RNAi) is a form of posttranscriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). In Drosophila melanogaster, the RNase III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purificati...

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Sonraí Bibleagrafaíochta
Main Authors: Ye, Xuecheng, Liu, Qinghua
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2008
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2855880/
https://ncbi.nlm.nih.gov/pubmed/18369775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-191-8_2
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