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Enzyme Amplified Array Sensing of Proteins in Solution and in Biofluids

We have developed an enzyme-nanoparticle sensor array where the sensitivity is amplified through enzymatic catalysis. In this approach cationic gold nanoparticles are electrostatically bound to an enzyme (β-galactosidase, β-Gal), inhibiting enzyme activity. Analyte proteins release the β-Gal, restor...

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Autors principals: Miranda, Oscar R., Chen, Hung-Ting, You, Chang-Cheng, Mortenson, David E., Yang, Xiao-Chao, Bunz, Uwe H. F., Rotello, Vincent M.
Format: Artigo
Idioma:Inglês
Publicat: 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2855490/
https://ncbi.nlm.nih.gov/pubmed/20329726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja1006756
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