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Identification of Inhibitors of V-ATPase Pumps in Yeast by HTS Flow Cytometry

Fluorescence intensity of the pH-sensitive carboxyfluorescein derivative BCECF was monitored by high throughput flow cytometry in living yeast cells. We measured fluorescence intensity of BCECF trapped in yeast vacuoles, acidic compartments equivalent to lysosomes where V-ATPases are abundant. Becau...

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Detalhes bibliográficos
Main Authors: Johnson, Rebecca M., Allen, Chris, Melman, Sandra D., Waller, Anna, Young, Susan M., Sklar, Larry A., Parra, Karlett J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2853757/
https://ncbi.nlm.nih.gov/pubmed/20018164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.020
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