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Identification of Inhibitors of V-ATPase Pumps in Yeast by HTS Flow Cytometry
Fluorescence intensity of the pH-sensitive carboxyfluorescein derivative BCECF was monitored by high throughput flow cytometry in living yeast cells. We measured fluorescence intensity of BCECF trapped in yeast vacuoles, acidic compartments equivalent to lysosomes where V-ATPases are abundant. Becau...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2853757/ https://ncbi.nlm.nih.gov/pubmed/20018164 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.020 |
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